71028
Monobromo(trimethylammonio)bimane bromide
suitable for fluorescence, ≥90.0% (HPLC)
동의어(들):
(Trimethylammonio)monobromobimane, 5-(Bromomethyl)-N,N,N,2,6-pentamethyl-1,7-dioxo-1H,7H-pyrazolo[1,2-a]pyrazole-3-methanaminium bromide, Brombimane q, Thiolye MQ, qBBr
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모든 사진(1)
About This Item
실험식(Hill 표기법):
C13H19Br2N3O2
CAS Number:
Molecular Weight:
409.12
Beilstein:
4630658
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.32
추천 제품
Quality Level
분석
≥90.0% (HPLC)
양식
powder
solubility
DMSO: 5 mg/mL
형광
λex 380 nm; λem 475 nm in 0.1 M phosphate pH 7.5
적합성
suitable for fluorescence
저장 온도
2-8°C
SMILES string
[Br-].CC1=C(CBr)N2N(C1=O)C(=O)C(C)=C2C[N+](C)(C)C
InChI
1S/C13H19BrN3O2.BrH/c1-8-10(6-14)15-11(7-17(3,4)5)9(2)13(19)16(15)12(8)18;/h6-7H2,1-5H3;1H/q+1;/p-1
InChI key
FCRPMMVPBVOKQS-UHFFFAOYSA-M
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생화학적/생리학적 작용
Monobromobimanes are essentially nonfluorescent until conjugated and readily react with low molecular weight thiols. Unlike common monobromobimanes, this carries a positive charge, thus permitting separation of conjugates by electrophoresis or cation-exchange chromatography
포장
Bottomless glass bottle. Contents are inside inserted fused cone.
기타 정보
Reagent for labeling thiols in biol. materials
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
S X Yuan et al.
FEBS letters, 234(1), 218-222 (1988-07-04)
The single cysteinyl residue 26 of spinach calmodulin was labelled with the thiol-specific bimane fluorescence probe. Following application of stoichiometric quantities of Ca2+ or aluminum ions to the protein, temperature-dependent fluorescence changes (anisotropy, lifetime) could be monitored via the label.
S S Ristow et al.
Immunological investigations, 14(5), 401-414 (1985-10-01)
Cell surface thiols are required for a line of cloned murine natural killer lymphocytes to bind to and lyse tumor target cells. These lymphocytes neither bound to nor killed YAC-1 or G1Tc cells when the effector lymphocyte cell surface thiols
M Balázs et al.
Biochemical and biophysical research communications, 140(3), 999-1006 (1986-11-14)
The accessibility of cell surface sulfhydryl groups in human peripheral lymphocytes was investigated with 5,5'-dithiobis-(2-nitrobenzoic acid) in the presence and absence of ionophore antibiotics and the monoclonal antibody, OKT-3. Only a few accessible protein thiols have been found on the
Claresta Joe-Wong et al.
Environmental science & technology, 46(18), 9854-9861 (2012-08-25)
Organic thiols are highly reactive ligands and play an important role in the speciation of several metals and organic pollutants in the environment. Although small thiols can be isolated and their concentrations can be estimated using chromatographic and derivatization techniques
J Lahav et al.
FEBS letters, 475(2), 89-92 (2000-06-20)
Cell adhesion is mediated by the integrin adhesion receptors. Receptor-ligand interaction involves conformational changes in the receptor, but the underlying mechanism remains unclear. Our earlier work implied a role for sulfhydryls in integrin response to ligand binding in the intact
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