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Merck
모든 사진(1)

주요 문서

56575

Sigma-Aldrich

GenElute-LPA

Neutral carrier for ethanol precipitation

동의어(들):

linear polyacrylamide

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.52

Quality Level

저장 온도

2-8°C

애플리케이션

GenElute-LPA has been used to:
  • extract DNA from cyanobacterial and algal cultures
  • precipitate genomic DNA
  • extract and purify DNA from snap-frozen uveal melanoma tissues
  • to extract RNA from isolated CD4+ cells

생화학적/생리학적 작용

The presence of LPA during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs, whereas most of the DNA is lost if no carrier is used. Very short DNA fragments, less than 20 base pairs, do not coprecipitate with LPA, allowing separation of labeled DNA from unreacted nucleotides by precipitation after the labeling reaction.

포장

GenElute LPA is tested for nucleases, and is supplied in nuclease-free water as a 25 mg/ml solution.

원리

GenElute LPA Linear Polyacrylamide is an efficient neutral carrier for precipitating picogram amounts of nucleic acids with ethanol. The nucleic acid precipitate can be collected simply by centrifugation. LPA offers several advantages for recovering DNA or studying DNA-protein interactions, relative to other carriers, such as tRNA or glycogen. tRNA interferes with DNA during phosphorylation with polynucleotide kinase and glycogen competes with protein in DNA-protein interaction studies. In contrast, LPA is completely inert. LPA is synthesized chemically and, therefore, is not contaminated with biological material. The precipitate is visible immediately upon addition of LPA, thus eliminating wait time and low temperature incubation.

법적 정보

GenElute is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Quantitative PCR enumeration of total/toxic Planktothrix rubescens and total cyanobacteria in preserved DNA isolated from lake sediments.
Savichtcheva O
Applied and Environmental Microbiology, 77(24), 8744-8753 (2011)
Peripheral immune responses in pregnant cattle following Neospora caninum infection.
Rosbottom A
Parasite Immunology, 29(4), 219-228 (2007)
Whole-genome microarray detects deletions and loss of heterozygosity of chromosome 3 occurring exclusively in metastasizing uveal melanoma.
Lake SL
Investigative Ophthalmology & Visual Science, 51(10), 4884-4891 (2010)
Promoter de-methylation of cyclin D2 by sulforaphane in prostate cancer cells.
Hsu A
Clinical epigenetics, 3, 3-3 (2011)
Ethanol precipitation of DNA with linear polyacrylamide as carrier.
C Gaillard et al.
Nucleic acids research, 18(2), 378-378 (1990-01-25)

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