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Merck
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Key Documents

51797

Sigma-Aldrich

Atto MB2 maleimide

Methylene Blue derivative, ≥90% (HPLC)

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About This Item

UNSPSC 코드:
12352111
NACRES:
NA.32

Quality Level

분석

≥90% (HPLC)
≥90% (degree of coupling)

형태

solid

제조업체/상표

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV 흡수

λ: 655-661 nm Amax

저장 온도

−20°C

애플리케이션

ATTO MB2 is a derivative of the well-known redox dye Methylene Blue. The dye can be reversibly reduced to the colorless leuko form. Upon oxidation the dye recovers and the absorption is fully restored. In common with most ATTO-labels the dye shows a high extinction coefficient. The dye is moderately hydrophilic. ATTO MB2 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides react more selectively with thiols. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

법적 정보

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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