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Merck
모든 사진(1)

주요 문서

5006

Sigma-Aldrich

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol

동의어(들):

Collagen Type I, PureCol Collagen

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About This Item

EC Number:
UNSPSC 코드:
12352202
NACRES:
NA.75

제품명

PureCol, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

생물학적 소스

bovine

Quality Level

무균

sterile-filtered

분석

99.9%

양식

lyophilized

포장

pkg of 15 mg

기술

cell culture | mammalian: suitable

결합 특이성

Peptide Source: Elastin

Peptide Source: Fibrinogen

배송 상태

wet ice

저장 온도

−20°C

일반 설명

PureCol collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol, lyophilized form is not recommended for the formation of a solid gel. PureCol collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

애플리케이션

PureCol has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

제조 메모

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

법적 정보

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Rebecca Long Heise et al.
Tissue engineering. Part A, 15(12), 3951-3960 (2009-07-03)
Successful approaches to tissue engineering smooth muscle tissues utilize biodegradable scaffolds seeded with autologous cells. One common problem in using biological scaffolds specifically is the difficulty of inducing cellular penetration and controlling de novo extracellular matrix deposition/remodeling in vitro. Our

문서

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

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