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Merck
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Key Documents

23479

Sigma-Aldrich

Nitrite/Nitrate Assay Kit, colorimetric

used for detection of nitric oxide metabolites

동의어(들):

Nitrate/Nitrite Assay Kit, colorimetric

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

Quality Level

사용

sufficient for 100 assays

적합성

passes test for application

저장 온도

2-8°C

애플리케이션

Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.

The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.

키트 구성품 전용

제품 번호
설명

  • NaNO2 Standard Solution (100 mM) 1 bottle

  • NaNO3 Standard Solution (100 mM) 1 bottle

  • Buffer solution (20 mM, pH 7.6) 1 bottle

  • Nitrate reductase (lyophilized) 1 bottle

  • Enzyme Co-factor (lyophilized) 1 bottle

  • Griess Reagent A 1 bottle

  • Griess Reagent B 1 bottle

관련 제품

제품 번호
설명
가격

픽토그램

CorrosionExclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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