106-05A

Human Dermal Fibroblasts: HDF, adult

• 동의어(들): HDF cells
• UNSPSC 코드: 41106514
• NACRES: NA.81

속성

• 생물학적 소스: human neonatal foreskin or adult skin (normal)
• Quality Level: 100
• 포장: pkg of 500,000 cells
• 제조업체/상표: Cell Applications, Inc
• 성장 모드: Adherent
• 핵형: 2n = 46
• 형태학: Fibroblast
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

일반 설명

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Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

세포주 기원

Skin

애플리케이션

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

성분

Fibroblast Basal Medium containing 10% FBS & 10% DMSO.

제조 메모

• Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
• Can be cultured at least 16 doublings.

배양 배지

Fibroblast Growth Medium (116-500) all-in-one ready to use media

계대배양 정규 작업

Please refer to the HDF Culture Protocol.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable