10112303

Shmac 5 cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human prostate, Epithelial

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human prostate
• 포장: tube of 5 μg 10112303-DNA-5UG; pkg of vial of cells 10112303-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: See Lang SH, et.al (2006)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer

설명

세포주 기원

Prostate cancer moderately well differentiated, early stage

포장

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA 프로파일

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11,12
D5S818: 12,14
D7S820: 7,12
THO1: 6,9.3
TPOX: 8,12
vWA: 15,17

배양 배지

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable