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Merck
모든 사진(1)

문서

32211

Sigma-Aldrich

Chloroform

puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

동의어(들):

Methylidyne trichloride, Trichloromethane

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About This Item

실험식(Hill 표기법):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein:
1731042
EC Number:
MDL number:
UNSPSC 코드:
12191502
PubChem Substance ID:
NACRES:
NA.04

Agency

USP/NF
reag. ISO
reag. Ph. Eur.

Quality Level

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

grade

puriss. p.a.

분석

99.0-99.4% (GC)

형태

liquid

포함

~1% ethanol as stabilizer

기술

RNA extraction: suitable

불순물

≤0.00001% free chlorine (Cl)
≤0.00005% free acid (as HCl)
≤0.0005% non-volatile matter
≤0.005% aldehydes and ketones (as CH3COCH3)
≤0.005% carbonyl compounds (as CO)
≤0.01% tetrachloroethene (GC)
≤0.01% tetrachloromethane (GC)
≤0.01% trichloroethene (GC)
≤0.01% water (Karl Fischer)
≤0.03% dichloromethane (GC)
0.6-1.0% ethanol (GC)

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.476-1.483 g/mL at 20 °C
1.492 g/mL at 25 °C (lit.)

음이온 미량물

chloride (Cl-): ≤1 mg/kg

양이온 미량물

Al: ≤0.5 mg/kg
B: ≤0.02 mg/kg
Ba: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.05 mg/kg
Sn: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES string

ClC(Cl)Cl

적합성

complies for appearance
complies for reaction against H2SO4
complies for suitability of determ. w. dithizone

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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일반 설명

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO2 aqueous suspensions (wavelength range of 310-380nm).

애플리케이션

Chloroform may be employed as a solvent for the following studies:
  • Extraction of RNA from plant cells.
  • Preparation of dioleoylphosphatidylcholine (DOPC) solution.
  • Dissolution of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).
It may be employed in a rapid quantitative method for the release of periplasmic proteins from bacterial strains.
Chloroform may be used as a solvent to enhance the enantioselectivity of secondary alcohols formed by the borohydride reduction of carbonyl compounds in the presence of optically active ketoiminatocobalt complexes.

기타 정보

For information on chloroform miscibility, please visit the following link:
Chloroform Miscibility/Immiscibility Table
The article number 32211-4X2.5L will be discontinued. Please order the single bottle 32211-2.5L which is physically identical with the same exact specifications.
The article number 32211-4X2.5L-M will be discontinued. Please order the single bottle 32211-2.5L-M which is physically identical with the same exact specifications.
The article number 32211-6X1L will be discontinued. Please order the single bottle 32211-1L which is physically identical with the same exact specifications.
The article number 32211-6X1L-M will be discontinued. Please order the single bottle 32211-1L-M which is physically identical with the same exact specifications.

픽토그램

Skull and crossbonesHealth hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

표적 기관

Central nervous system, Liver,Kidney

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


시험 성적서(COA)

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문서 라이브러리 방문

Chloroform is not solvent but activator for cobalt complex catalyst of enantioselective borohydride reduction.
Kokura, Ai, et al.
Chemistry Letters (Jpn), 36(1), 26-27 (2006)
Gurman Singh Pall et al.
Nucleic acids research, 35(8), e60-e60 (2007-04-05)
The northern blot, or RNA gel blot, is a widely used method for the discovery, validation and expression analysis of small regulatory RNA such as small interfering RNA (siRNA), microRNA (miRNA) and piwi-interacting RNA (piRNA). Although it is straightforward and
G F Ames et al.
Journal of bacteriology, 160(3), 1181-1183 (1984-12-01)
We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the
Jameel A Feshitan et al.
Journal of colloid and interface science, 329(2), 316-324 (2008-10-28)
Microbubbles used as contrast agents for ultrasound imaging, vectors for targeted drug delivery and vehicles for metabolic gas transport require better size control for improved performance. Mechanical agitation is the only method currently available to produce microbubbles in sufficient yields
Z V Leonenko et al.
Biochimica et biophysica acta, 1509(1-2), 131-147 (2000-12-19)
We have used magnetic alternating current mode atomic force microscopy (MAC-AFM) to investigate the formation of supported phospholipid bilayers (SPB) by the method of vesicle fusion. The systems studied were dioleoylphosphatidylcholine (DOPC) on mica and mica modified with 3-aminopropyl-triethoxy-silane (APTES)

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