PPB019

Bis-Tris Bicine EDTA buffer

pH 7.2, pHast Pack^™, powder

• 동의어(들): 1X Bis-Tris transfer buffer, Bis-Tris
• UNSPSC 코드: 41105319
• NACRES: NA.25

속성

• 제품 라인: pHast Pack^™
• Quality Level: 100
• 양식: powder
• 저장 조건: (Tightly closed. Dry)
• pH: 7.2
• 양이온 미량물: Pb: ≤5 ppm
• 외래 활성: DNAse, free; NICKase, free; Protease, free; RNAse, free
• 저장 온도: room temp

설명

일반 설명

Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.

애플리케이션

Bis-Tris buffer may be used in western blot as a transfer buffer.

특징 및 장점

• Ready-to-use buffer
• No measuring and pH adjustment needed
• Certified quality for reproducibility
• Minimizes exposure to toxic chemicals
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

포장

Foil pouches

재구성

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

저장 및 안정성

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

기타 정보

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법적 정보

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H318
• 예방조치 성명서: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3