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Merck
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문서

D9015

Sigma-Aldrich

3,3′-Diaminobenzidine tetrahydrochloride hydrate

≥96% purity (TLC), powder

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About This Item

Linear Formula:
C12H14N4 · 4HCl · xH2O
CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

3,3′-Diaminobenzidine tetrahydrochloride hydrate, ISOPAC®

Quality Level

분석

≥96% (TLC)

형태

powder

색상

off-white to faint red, to beige

mp

280 °C

solubility

water: 50 mg/mL, clear to slightly hazy

응용 분야

diagnostic assay manufacturing
hematology
histology

저장 온도

room temp

SMILES string

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

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애플리케이션

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.

생화학적/생리학적 작용

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

포장

Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.

주의사항

Injecting a compatible solvent permits preparation of any desired strength solution without exposure.

재구성

Dissolving the contents in 100 mL of solvent yields a 0.1% solution.

법적 정보

Isopac is a registered trademark of Merck KGaA, Darmstadt, Germany

ISOPAC

제품 번호
설명
가격

픽토그램

Health hazardExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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