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Merck
모든 사진(3)

Key Documents

A3643

Sigma-Aldrich

Agarose

Type IV, Special High EEO

동의어(들):

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
41105317
PubChem Substance ID:
NACRES:
NA.21

생물학적 소스

algae (marine)

Quality Level

유형

Type IV

형태

powder

기술

electrophoresis: suitable

EEO

≥0.3

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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애플리케이션

Agarose has been used in the electrophoresis of RNA for northern blot. It has also been used in coating microscope slides for counter immunoelectrophoresis.

품질

Characterized by lower sulfate content and lower non-specific protein binding capacity than Type III. Trailing and smearing of proteins due to non-specific protein binding is minimized.

분석 메모

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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