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Merck
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Key Documents

A2576

Sigma-Aldrich

Agarose

Ultra-low Gelling Temperature, molecular biology grade

동의어(들):

3,6-Anhydro-α-L-galacto-β-D-galactan

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
41105317
PubChem Substance ID:
NACRES:
NA.21

Grade

Molecular Biology

Quality Level

유형

Type IX-A

형태

powder

기술

electrophoresis: suitable

불순물

≤7% water

≤0.4%

EEO

≤0.12

mp

≤62 °C

전이 온도

gel point ≤20 °C (1.5% gel)

겔 강도

≥300 g/cm2 (1.5% gel)

음이온 미량물

sulfate (SO42-): ≤0.14%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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일반 설명

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically.

애플리케이션

Agarose has been used:
  • in thaumatin and elastase protein crystallization
  • in microcasting using a polydimethylsiloxane (PDMS) mold with micro-channel
  • in agarose micro-confinement array

Agarose is super low melting point and recommended for use in capillary electrophoresis, tissues & cell culture, and viral plaque assay.
Yields unusually strong gels for an ultra-low gelling agarose. Ideal for electrophoresis of heat-labile samples and for growth of hybridomas or other cell lines.

분석 메모

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

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Japanese Journal of Applied Physics, 48(7R), 075502-075502 (2009)
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