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Key Documents

SFABIKB

Roche

KAPA SYBR® FAST

suitable for qPCR, 2 ×, ABI PRISM®

동의어(들):

qPCR

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About This Item

UNSPSC 코드:
41106300
NACRES:
NA.55

Quality Level

사용

sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions
sufficient for 5000 reactions

유통기한

≤12 mo.

특징

dNTPs included
hotstart

포장

kit of 1 mL (100 x 20 μL rxn; KK4603)
kit of 10 mL (1000 x 20 μL rxn; KK4605)
kit of 5 mL (500 x 20 μL rxn; KK4604)
kit of 50 mL (5000 x 20 μL rxn; KK4617)

제조업체/상표

Roche

농도

2 ×

기술

qPCR: suitable

입력

purified DNA

호환성

for use with Applied Biosystems® 5700
for use with Applied Biosystems® 7000
for use with Applied Biosystems® 7300
for use with Applied Biosystems® 7700
for use with Applied Biosystems® 7900HT
for use with StepOne
for use with StepOnePlus

검출 방법

SYBR® Green

저장 온도

−20°C

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일반 설명

KAPA SYBR® FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.

The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.

애플리케이션

KAPA SYBR® FAST qPCR kit can be used for:
  • Gene expression
  • Gene knockdown validation
  • Microarray validation
  • ChIP analysis
  • Low copy detection
  • Absolute quantification of NGS libraries
  • Real-time PCR

특징 및 장점

Quantitate changes in gene expression more accurately
  • High reaction efficiency between 95 – 105% improves accuracy and reproducibility.
  • Unbiased efficiency across a wide range of GC contents and amplicon lengths.

Detect low copy and difficult targets consistently
  • Improved processivity results in earlier Cq scores.
  • Higher fluorescence detection across varying AT- and GC-rich targets.
  • Novel enzyme is resistant to SYBR® inhibition.

Complete real-time PCR runs in just 40 minutes
  • 55% shorter run times with fast cycling protocol.
  • Maintain high performance when switching from standard to fast protocols.

Quick Notes:
  • This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
  • The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
  • 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
  • For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
  • Do not exceed 25 μL reaction volumes.

품질

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.

제조 메모

Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C.

The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) ABI PRISM® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.

기타 정보

For Research Use Only. Not for use in diagnostic procedures.

법적 정보

ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

키트 구성품 전용

제품 번호
설명

  • KAPA SYBR® FAST DNA Polymerase

  • reaction buffer

  • dNTPs

  • SYBR® Green I dye

  • MgCl2 2.5 mM

픽토그램

Health hazard

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

STOT SE 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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