추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
12CA5, monoclonal
분석
>90% (HPLC and SDS-PAGE)
형태
frozen liquid (11666606001)
lyophilized (11583816001)
포장
pkg of 200 μg (11583816001)
pkg of 5 mg (11666606001 [1 ml])
제조업체/상표
Roche
농도
5 mg/ml (11666606001)
동형
IgG2bκ
에피토프 서열
YPYDVPDYA
저장 온도
−20°C
일반 설명
Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.
특이성
Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
면역원
Amino acids 98-106 from the human influenza virus hemagglutinin protein.
애플리케이션
Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
- Dot blots
- Immunochemistry
- Immunoprecipitation
- Western blotting
생화학적/생리학적 작용
Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.
품질
Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.
제조 메모
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- Dot blot: 0.1 to 1 μg/ml
- Immunfluorescence: 1-10 μg/ml
- Western blot: 0.1 to 1 μg/ml
재구성
11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.
기타 정보
For life science research only. Not for use in diagnostic procedures.
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Storage Class Code
13 - Non Combustible Solids
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus.
Stevens J, et al.
Science, 312(5772), 404-410 (2006)
On the origin of the human influenza virus subtypes H2N2 and H3N2
Scholtissek C, et al.
Virology, 87(1), 13-20 (1978)
Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin.
Skehel J J and Wiley D C
Annual Review of Biochemistry, 69(1), 531-569 (2000)
Jen-Liang Su et al.
Cancer research, 71(21), 6878-6887 (2011-09-14)
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Hsu-Ping Kuo et al.
Biochemical and biophysical research communications, 389(4), 640-644 (2009-09-22)
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