추천 제품
Quality Level
사용
sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions
유통기한
≤12 mo.
특징
dNTPs included
hotstart
포장
kit of 1 mL (100 x 20 μL rxn; KK4716)
kit of 10 mL (1000 x 20 μL rxn; KK4718)
kit of 5 mL (500 x 20 μL rxn; KK4717)
pkg of
제조업체/상표
Roche
농도
2 ×
기술
qPCR: suitable
입력
purified DNA
호환성
for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems® 7500
for use with Applied Biosystems® ViiA 7
for use with QuantStudio™
검출 방법
probe-based
저장 온도
−20°C
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일반 설명
애플리케이션
- Genotyping
- Gene expression analysis
- Multiplex qPCR
- SNP genotyping/allelic discrimination
- Microarray validation
- Real time(RT)-quantitative polymerase chain reaction (qPCR)/qPCR
특징 및 장점
- Exceptional differentiation of heterozygous and homozygous alleles
- Discrete clusters in SNP genotyping assays
Achieve excellent reproducibility and efficiency:
- Fast, high performance five-colored multiplex PCR
- Similar abundance levels achieved with low copy number
- Broad dynamic range of up to 10 orders of magnitude
Increase flexibility:
- Compatible with both standard and fast cycling protocolsRetain accurate quantification
Quick Notes:
- These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
- Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
- For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
- For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
- Do not exceed 25 μL volumes.
품질
제조 메모
KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.
기타 정보
법적 정보
키트 구성품 전용
- KAPA PROBE FAST qPCR Master Mix (2X) with ROX™ Low
- KAPA Taq HotStart® DNA Polymerase
- reaction buffer
- dNTPs
- MgCl2 5 nM
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
문서
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.