추천 제품
생물학적 소스
yeast (Candida boidini)
Quality Level
재조합
expressed in E. coli
형태
lyophilized
특이 활성도
~0.4 units/mg protein (At 25 °C with formate as the substrate.)
분자량
74 kDa
포장
pkg of 250 U (10837016001)
pkg of 80 U (10244678001)
제조업체/상표
Roche
농도
≥10-20 % (w/w)
기술
activity assay: suitable
색상
off-white
최적 pH
7.5-8.5
solubility
water: soluble 10 g/L
적합성
suitable for gel electrophoresis
UniProt 수납 번호
응용 분야
life science and biopharma
외래 활성
ADH <0.0500%
LDH <0.0500%
MDH <0.1000%
저장 온도
2-8°C
관련 카테고리
일반 설명
Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
특이성
Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.
애플리케이션
Most widely used in cofactor recycling systems for NADH.
품질
Contaminants: <0.005% LDH and ADH each, <0.1% MDH
단위 정의
One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.
저장 및 안정성
Store dry
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Katja Schirwitz et al.
Protein science : a publication of the Protein Society, 16(6), 1146-1156 (2007-05-26)
The understanding of the mechanism of enzymatic recovery of NADH is of biological and of considerable biotechnological interest, since the essential, but expensive, cofactor NADH is exhausted in asymmetric hydrogenation processes, but can be recovered by NAD(+)-dependent formate dehydrogenase (FDH).
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
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