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Key Documents

CSPD-RO

Roche

CSPD ready-to-use

Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate

동의어(들):

ready-to-use, cspd

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About This Item

UNSPSC 코드:
41105500

Quality Level

100

분석

>98% (HPLC)

형태

solution

분자량

Mr 461

포장

pkg of 1 mL (11655884001[25mM])
pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use)

제조업체/상표

Roche

저장 온도

2-8°C

일반 설명

CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

애플리케이션

CSPD ready-to-use has been used for:
  • the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)
  • colony and plaque hybridization
  • gel shift assay
  • sequencing
It may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports.

특징 및 장점

  • Save time with a fast, highly sensitive substrate.
  • Easily strip and reprobe membranes.
  • Take multiple exposures of up to two days for each experiment.
  • Reuse the substrate by filtering and storing in sodium azide.

물리적 형태

25mM solution (11.6mg/ml), 100x concentrated, colorless solution.

제조 메모

Storage conditions (working solution): Washing Buffer: 15 to 25 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C

기타 정보

For life science research only. Not for use in diagnostic procedures.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°C)

does not flash

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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