추천 제품
유통기한
≤12 mo.
Quality Level
포장
kit of 1.25 mL (100 x 25 μL rxn; KK5801)
kit of 6.25 mL (500 x 25 μL rxn; KK5802)
제조업체/상표
Roche
농도
2 ×
기술
PCR: suitable
저장 온도
−20°C
일반 설명
KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzyme derived through a process of directed evolution. KAPA2G FAST HotStart® DNA Polymerase is an antibody-mediated hot start formulation engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes, allowing for more uniform multiplexed PCR.
KAPA2G Fast HotStart PCR Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance and does not require specialized PCR consumables or thermocyclers.
KAPA2G Fast HotStart PCR Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance and does not require specialized PCR consumables or thermocyclers.
애플리케이션
KAPA2G Fast Multiplex Mix has been used for:
- Typing of transgenic organisms
- Amplification of microsatellites
- Typing and detection of pathogens
- Amplification of multiple DNA fragments for single nucleotide polymorphism (SNP) genotyping
- Semi-quantitative PCR
- 3-plex PCR
생화학적/생리학적 작용
DNA fragments generated with KAPA2G Fast DNA Polymerase have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase, and may be used for sequencing, restriction enzyme digestion and cloning. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. PCR products generated with KAPA2G Fast are 3′-dA-tailed and may be cloned into TA cloning vectors.
특징 및 장점
Improve sensitivity, specificity, and yields
Increase speed without compromising performance
Quick Notes:
- Uniform representation of all amplicons
- Successful multiplex PCR with difficult, GC-rich targets
Increase speed without compromising performance
- 60% reduction in cycling time
- Extension times as low as 15 seconds
Quick Notes:
- KAPA2G Fast Multiplex Mix contains the engineered KAPA2G Fast HotStart DNA Polymerase, for fastand efficient multiplex PCR.
- The KAPA2G Fast Multiplex Mix contains a buffer optimized for multiplex PCR, with 0.2 mM of each dNTP and 3 mM MgCl2 (at 1X).
- Use 0.2 μM of each primer, and 10–100 ng of template DNA per reaction.
- Anneal at 60°C for 30 seconds.
- Perform extension for 15 sec, and increase for longer amplicons, and/or highly multiplexed reactions.
품질
Each batch of KAPA2G Fast HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (AgilentProtein 230 Assay). KAPA2G Fast Multiplex PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contaminationlevels.
제조 메모
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the ReadyMix has been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.
기타 정보
For Research Use Only. Not for use in diagnostic procedures.
법적 정보
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
키트 구성품 전용
제품 번호
설명
- KAPA2G Fast HotStart® DNA Polymerase
- Reaction buffer
- dNTPs
- MgCl2 3 mM
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Oral - STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Pellets of proof: First glimpse of the dietary composition of adult odonates as revealed by metabarcoding of feces.
Ecology and Evolution, 7(20), 8588-8598 (2017)
Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering.
Nature Protocols, 9(10), 2285-2285 (2014)
문서
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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