콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

11815016001

Roche

Anti-HA Affinity Matrix

from rat IgG1

동의어(들):

affinity matrix, anti-ha

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203

생물학적 소스

rat

Quality Level

결합

agarose conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

3F10, monoclonal

형태

slurry

포장

pkg of 1 mL (settled resin volume)

제조업체/상표

Roche

동형

IgG1

에피토프 서열

YPYDVPDYA

용량

2-8 nmol/mL binding capacity

저장 온도

2-8°C

일반 설명

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)

특이성

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).

면역원

Amino acids 98-106 from the human influenza virus hemagglutinin protein

애플리케이션

Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
  • Western blotting using the Anti-HA antibody
  • Silver staining (or similar protein stain)
  • In coimmunoprecipitation.
Use Anti-HA Affinity Matrix for:
  • Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts
  • Affinity column purification of HA-tagged proteins from crude protein extracts

특징 및 장점

  • Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
  • Use nondenaturing conditions for elution of your HA-tagged protein of choice.
  • Purify even rarely expressed HA-tagged proteins.
  • Highly specific to YPYDVPDYA.
  • Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
  • Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.

품질

Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.

물리적 형태

1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.

분석 메모

Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.

기타 정보

For life science research only. Not for use in diagnostic procedures.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Francisco José Taberner et al.
BMC cell biology, 11, 47-47 (2010-07-01)
The control of the subcellular localization of cell cycle regulators has emerged as a crucial mechanism in cell division regulation. The active transport of proteins between the nucleus and the cytoplasm is mediated by the transport receptors of the beta-karyopherin
Geetha Hewawasam et al.
Molecular cell, 40(3), 444-454 (2010-11-13)
Cse4 is a variant of histone H3 that is incorporated into a single nucleosome at each centromere in budding yeast. We have discovered an E3 ubiquitin ligase, called Psh1, which controls the cellular level of Cse4 via ubiquitylation and proteolysis.
Abdirahman Abdi et al.
Cellular and molecular life sciences : CMLS, 67(19), 3355-3369 (2010-06-29)
Over the last decade, several protein kinases inhibitors have reached the market for cancer chemotherapy. The kinomes of pathogens represent potentially attractive targets in infectious diseases. The functions of the majority of protein kinases of Plasmodium falciparum, the parasitic protist
David A Hubert et al.
The EMBO journal, 22(21), 5679-5689 (2003-11-01)
The Arabidopsis protein RPM1 activates disease resistance in response to Pseudomonas syringae proteins targeted to the inside of the host cell via the bacterial type III delivery system. We demonstrate that specific mutations in the ATP-binding domain of a single
Stephen C Kales et al.
PloS one, 9(1), e87116-e87116 (2014-01-28)
The Cbl proteins (Cbl, Cbl-b, and Cbl-c) are a highly conserved family of RING finger ubiquitin ligases (E3s) that function as negative regulators of tyrosine kinases in a wide variety of signal transduction pathways. In this study, we identify a

프로토콜

Anti-HA Affinity Matrix Protocol & Troubleshooting

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.