추천 제품
사용
sufficient for 96 reactions
제조업체/상표
Roche
배송 상태
dry ice
저장 온도
−20°C
특이성
The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.
애플리케이션
For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.
특징 및 장점
Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
- High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
- Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
- Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
- Fast results: The entire procedure can be performed in one day
- Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing
포장
1 kit containing 14 components.
서열
Chain Length Positive control: 520 bp.
기타 정보
For life science research only. Not for use in diagnostic procedures.
키트 구성품 전용
제품 번호
설명
- Lysis Reagent
- Neutralization Reagent
- PCR Mix ready-to-use
- Denaturation Reagent
- Hybridization Buffer
- Biotinylated Capture Probe
- Washing Buffer 10x concentrated
- Anti-Digoxigenin-POD antibody
- Conjugate Dilution Buffer
- TMB Substrate Solution
- Stop Reagent
- Positive Control DNA
- Streptavidin-coated Microplate
- Adhesive Plate Cover Foils
모두 보기 (14)
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Novel Non-phosphorylated Serine 9/21 GSK3?/? Antibodies: Expanding the Tools for Studying GSK3 Regulation
Grabinski T, et al.
Frontiers in Molecular Neuroscience, 9, 123-123 (2016)
Novel Non-phosphorylated Serine 9/21 GSK3?/a Antibodies: Expanding the Tools for Studying GSK3 Regulation
Tessa G, et al.
Frontiers in Molecular Neuroscience (2016)
Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi
Margaryan N V, et al.
International Journal of Molecular Sciences, 17(3), 418-418 (2016)
Behshad Pournasr et al.
Stem cell research, 48, 101953-101953 (2020-08-22)
Propionic acidemia (PA) is an autosomal recessive metabolic liver disease caused by defects in propionyl CoA carboxylase. Propionyl CoA carboxylase is a dodecameric enzyme consisting of multiple copies of alpha and beta subunits encoded by the PCCA and PCCB genes.
Sm Nashir Udden et al.
eLife, 8 (2019-04-17)
Hepatocellular carcinoma (HCC) is a deadly human cancer associated with chronic inflammation. The cytosolic pathogen sensor NLRP12 has emerged as a negative regulator of inflammation, but its role in HCC is unknown. Here we investigated the role of NLRP12 in
문서
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
프로토콜
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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