추천 제품
형태
solution
Quality Level
포장
pkg of 2 μg
제조업체/상표
Roche
환경친화적 대안 제품 특성
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
환경친화적 대안 카테고리
저장 온도
−20°C
일반 설명
This antisense RNA probe (human β-actin) is in vitro transcribed in the presence of digoxigenin (DIG)-UTP. The transcript has a length of 588 bases. 550 bases are complementary to the 5′ region of human β-actin mRNA (nucleotides 69 to 6l8, EMBL: HSAC07). The additional 25 bases at the 5′ end, and the 13 additional bases at the 3′ end of the transcript are specific for the promoter/polylinker region of the transcription vector.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
애플리케이션
Actin RNA Probe, DIG-labeled, is specifically useful for evaluating the quality and quantity of various RNA species and can be used:
- in In situ hybridization (for example, as a control in mRNA detection)
- for quality control in the construction of cDNA libraries
- in northern blot analysis to evaluate RNA from various human cell lines and tissue samples
성분
Actin RNA Probe, DIG-labeled, is supplied in autoclaved, DEPC-treated water.
서열
Agarose gel electrophoresis under denaturing conditions and subsequent northern blot analysis reveal a defined band of 588 bases.
제조 메모
Working solution: Dilute Sample
Storage conditions (working solution): During assay, the preparation should be maintained at 0 °C.
- Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.
The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification. - Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.
Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody
concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.
Storage conditions (working solution): During assay, the preparation should be maintained at 0 °C.
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
No data available
Flash Point (°C)
No data available
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Journal of dental research, 81(11), 771-775 (2002-10-31)
The elastic system fibers consist of three types--oxytalan, elaunin, and elastic fibers--differing in their relative microfibril and elastin contents. All three types are found in human gingiva, but human periodontal ligaments contain only elastin-free fibers. We examined cultured human gingival
문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.