11427598910

Biotin-16-ddUTP

≥98% (HPLC), solution, 1 mM, suitable for hybridization

• 동의어(들): Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate
• 실험식(Hill 표기법): C₃₂H₅₂N₇O₁₇P₃S
• CAS Number: 188755-42-2
• Molecular Weight: 931.78
• UNSPSC 코드: 41116100

속성

• Quality Level: 100
• 분석: ≥98% (HPLC)
• 형태: solution
• 분자량: 955.5 (biotin-16-ddUTP-Li₄)
• 포장: pkg of 25 μL (25 nmol; 1mM)
• 제조업체/상표: Roche
• 농도: 1 mM
• 기술: hybridization: suitable
• 저장 온도: −20°C

설명

일반 설명

Biotin-16-ddUTP, tetralithium salt; 1mM

애플리케이션

• Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
• DNA polymerase I (Holoenzyme and Klenow fragment)
• T4 DNA polymerase
• Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).

Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA.

• The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
• Colony and plaque screening
• In situ hybridization

In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.

특징 및 장점

Biotin is bound to dideoxyuridine triphosphate via an amide linkage.

품질

Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash