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11388908910

Roche

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

동의어(들):

Biotin-16-UTP, biotin

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모든 사진(1)

About This Item

UNSPSC 코드:
41116100

분석

97.7% (HPLC)

Quality Level

100

형태

solution

분자량

Mr 987.5 (biotin-16-UTP-Li4)

포장

pkg of 25 μL (250 nmol; 10mM)

제조업체/상표

Roche

색상

colorless

solubility

water: miscible

배송 상태

dry ice

저장 온도

−20°C

일반 설명

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

애플리케이션

Biotin-labeled RNA can be used as a hybridization probe for:
  • Northern blots
  • Southern blots
  • Plaque or colony lifts
  • RNase protection experiments
  • In situ hybridization
  • Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

품질

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

물리적 형태

Biotin-16-UTP, tetralithium salt, 10mM solution

기타 정보

For life science research only. Not for use in diagnostic procedures.

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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