11426346910

Anti-Fluorescein-POD, Fab fragments

from sheep

• 동의어(들): antibody
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: sheep
• Quality Level: 100
• 결합: peroxidase conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: lyophilized (stabilized)
• 포장: pkg of 150 U
• 제조업체/상표: Roche
• 저장 온도: 2-8°C

설명

일반 설명

Fab fragments from polyclonal anti-fluorescein antibodies, conjugated to horseradish peroxidase. The reagent is an anti-fluorescein antibody, Fab fragments from sheep, conjugated with horse-radishperoxidase (POD). After immunization with fluorescein the sheep IgG (immunoglobulin G) was purified by ion exchange chromatography and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated with POD and stabilized in 60mM Tris-Hepes-buffer, 0.4% bovine immunoglobulin (w/v), 0.2% Germall II (w/v), pH 7.2.

특이성

The polyclonal antibody reacts with free and bound fluorescein.

애플리케이션

Anti-Fluorescein-POD, Fab fragments are used for detection of fluorescein-labeled compounds in:

• Dot blot
• ELISA (enzyme-linked immunosorbent assay)
• Immunohistocytochemistry
• In situ hybridization
• Southern blot
• Western blot

제조 메모

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:

• Dot blot: 150 mU/ml
• ELISA: 50 to 150 mU/ml
• Immunohistocytochemistry: 250 to 500 mU/ml
• In situ hybridization: 1.5 to 7.5 U/ml
• Southern blot: 150 mU/ml
• Western blot: 500 to 1000 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

재구성

Add 1 ml double-distilled water to a final concentration of 150 U/ml.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash