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Merck
모든 사진(1)

문서

10634425001

Roche

n-Octylglucoside

non-ionic

동의어(들):

Octyl β-D-glucopyranoside, n-Octyl glucoside, OGP

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About This Item

실험식(Hill 표기법):
C14H28O6
CAS Number:
Molecular Weight:
292.37
Beilstein:
84118
MDL number:
UNSPSC 코드:
12352200
PubChem Substance ID:

분석

99% (GC)

분자량

micellar avg mol wt 25,000
292.4

포장

pkg of 10 g

제조업체/상표

Roche

응집 번호

84

기술

dialysis: suitable

CMC

20-25 mM (20-25°C)

전이 온도

cloud point >100 °C

배송 상태

ambient

SMILES string

CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O

InChI

1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1

InChI key

HEGSGKPQLMEBJL-RKQHYHRCSA-N

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일반 설명

1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.

애플리케이션

N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.
Non-ionic, dialyzable detergent for the solubilization and isolation of membrane proteins. Has been shown to increase the resolution of proteins in 2D gels.

품질

Purity: 99% (from C), homogeneous (GC)

Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C14H28O6

제조 메모

Working concentration: 46 mM
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.

저장 및 안정성

Store at 15 to 25 °C. (Store dry!)

기타 정보

For life science research only. Not for use in diagnostic procedures.

시험 성적서(COA)

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문서 라이브러리 방문

Howard R Mellor et al.
The Biochemical journal, 374(Pt 2), 307-314 (2003-05-29)
The N-alkyl moiety of N-alkylated imino sugars is crucial for therapeutic activities of these compounds as inhibitors of glycosphingolipid (GSL) biosynthesis and as antivirals. The improved potency afforded by a long N-alkyl moiety is coincident with increased compound-induced cytotoxicity. Therefore
W A Petri et al.
The Journal of biological chemistry, 254(11), 4313-4316 (1979-06-10)
The glycoprotein of vesicular stomatitis (VS) virus was selectively liberated from the virion membrane by the dialyzable nonionic detergent, beta-D-octylglucoside. The isolated viral glycoprotein could be rendered virtually free of phospholipid and detergent, under which conditions it formed tail-to-tail glycoprotein
Olgica Trenchevska et al.
Journal of proteome research, 9(11), 5969-5973 (2010-09-09)
Protein biomarkers are essential in assessing pathogenic processes. The impetus for finding new biomarkers has been accelerated by the arrival of the "omics" technologies. However, equally important is the rediscovery of existing biomarkers with these new approaches as novel variants
Olgica Trenchevska et al.
Proteome science, 9(1), 19-19 (2011-04-12)
Post-translational modifications and genetic variations give rise to protein variants that significantly increase the complexity of the human proteome. Modified proteins also play an important role in biological processes. While sandwich immunoassays are routinely used to determine protein concentrations, they

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