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Merck
모든 사진(1)

주요 문서

10108987001

Roche

Pyrophosphatase, inorganic (PPase)

from yeast

동의어(들):

inorganic pyrophosphatase

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About This Item

효소 위원회 번호:
UNSPSC 코드:
12352204

생물학적 소스

yeast

Quality Level

양식

suspension

특이 활성도

~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)

포장

pkg of 1 mL (1 mg)

제조업체/상표

Roche

저장 온도

2-8°C

일반 설명

Pyrophosphate phosphohydrolase
The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.

특이성

Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.

애플리케이션

Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.
Pyrophosphatase, inorganic (PPase) has been used in :
  • Enzymatic nitric oxide (NO) detection system
  • In vitro transcription
  • Multiply-primed rolling circle amplification

생화학적/생리학적 작용

Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.

품질

Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).

물리적 형태

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

기타 정보

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

No data available

Flash Point (°C)

No data available


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문서 라이브러리 방문

Michael A Kron et al.
Protein science : a publication of the Protein Society, 17(6), 983-989 (2008-04-16)
Diagnosis of eukaryotic parasitic infection using antibody-based tests such as ELISAs (enzyme-linked immunosorbent assays) is often problematic because of the need to differentiate between homologous host and pathogen proteins and to ensure that antibodies raised against a peptide will also
Tracking the m7G-cap during translation initiation by crosslinking methods.
Gross L, et al.
Methods, 137, 3-10 (2018)
2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO.
Picard-Jean F, et al.
PLoS ONE, 13(3), e0193804-e0193804 (2018)
Yakov Y Woldman et al.
Free radical biology & medicine, 47(10), 1339-1345 (2009-09-16)
Nitric oxide (NO) is a free radical involved in many physiological processes including regulation of blood pressure, immune response, and neurotransmission. However, the measurement of extremely low, in some cases subnanomolar, physiological concentrations of nitric oxide presents an analytical challenge.
Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis.
Kang Y, et al.
Nature Protocols, 10(7), 974-974 (2015)

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