SAE0215

Inorganic Pyrophosphatase from Escherichia coli

≥100 units/mL, buffered aqueous solution

• UNSPSC 코드: 12352204

속성

• 생물학적 소스: Escherichia coli
• Quality Level: 200
• 재조합: expressed in E. coli
• Grade: for molecular biology
• 설명: Recombinant, expressed in E.coli
• 분석: ≥95% (size exclusion chromatography)
• 양식: buffered aqueous solution
• 특이 활성도: ≥100 units/mL
• 유통기한: 2 yr at -20 °C ((retest))
• 분자량: 19.7 kDa
• 저장 조건: OK to freeze
• 농도: ≥100 units/mL
• 색상: colorless
• 최적 pH: 9.0 (25 °C)
• pH: 8.0 (25 °C)
• solubility: soluble; water: miscible
• 적합성: suitable for molecular biology
• UniProt 수납 번호: P0A7A9
• 응용 분야: research use
• 외래 활성: DNAse, none detected; RNAse, none detected; Nickase, none detected
• 배송 상태: dry ice
• 저장 온도: -10 to -25°C

설명

일반 설명

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate hydrolysis. It plays an important role in energy metabolism by providing a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis. Nucleic acid synthesis would be energetically impossible in vivo if not coupled with the hydrolysis of pyrophosphate (PPi).

애플리케이션

This product is based on the native pyrophosphatase from E. coli, Uniprot No. P0A7A9. Pyrophosphatase in E. coli is a homohexameric protein containing 175 amino acids residues per subunit. This product is a recombinant protein expressed in E. coli and induced by IPTG. Each subunit has a MW of 19.7 kDa and theoretical pI of ~5. The protein activity is Mg2+ dependent and it is a relatively thermostable protein.

생화학적/생리학적 작용

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate to phosphate. It plays an important role in energy metabolism as it provides a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis.

특징 및 장점

This product has a purity minimum of 95% (SEC-HPLC) and an activity minimum of 100 units/mL to enhance RNA yield during transcription.

단위 정의

One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 9 at 25 °C. The reaction buffer used for determination of enzyme activity contains 50 mM Tris-HCl, pH 9.0.

물리적 형태

The product is supplied as an aqueous solution containing 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% glycerol, titrated to pH 8 at 25 °C.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1