추천 제품
생물학적 소스
Bacillus polymyxa
Quality Level
양식
lyophilized
특이 활성도
≥10 units/mg protein (20 U/vial (37 °C, casein as substrate, pH 7.5))
포장
pkg of 10 × ~2 mg
제조업체/상표
Roche
농도
0.6-2.4 U/mL
기술
tissue processing: suitable
색상
white
최적 pH
6.0-8.5
solubility
water: soluble
응용 분야
life science and biopharma
sample preparation
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact.
특이성
Dispase® is a non-specific metalloprotease.
애플리케이션
Dispase® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.
특징 및 장점
- Rapid, effective, yet gentle agent that liberates intact cells
- Maintains cell membrane integrity
- Non-mammalian source - free of mycoplasma and animal virus contamination
- Extremely stable to influences of temperature, pH, and interference by serum components
- Easily inactivated by chelating agents or by dilution
- Delivers higher activity and convenience
Contents
10 vials with approximately 2mg each (= 20U/vial).
규격
Enzyme activity: =10U/mg (+37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
단위 정의
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides from casein corresponding to 1 μmol (181 μg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of RAS units of Dispase® with those cited in the Japanese literature (where concentrations of 1,000 to 2,000 Dispase® units /ml are not uncommon) suggests one Roche Applied Science unit of Dispase® I equals approximately 416 Japanese units of Dispase®.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 μg tyrosine per min and ml at pH 7.5 and +37 °C.
A practical comparison of RAS units of Dispase® with those cited in the Japanese literature (where concentrations of 1,000 to 2,000 Dispase® units /ml are not uncommon) suggests one Roche Applied Science unit of Dispase® I equals approximately 416 Japanese units of Dispase®.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 μg tyrosine per min and ml at pH 7.5 and +37 °C.
물리적 형태
white lyophilizate, before lyophilization filtered through 0.2 μm pore size membrane
제조 메모
Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® I enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months, the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing.
The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® I enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months, the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing.
The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
저장 및 안정성
Store at 2 to 8 °C. (Store dry!)
기타 정보
For life science research only. Not for use in diagnostic procedures.
법적 정보
Dispase is a registered trademark of Godo Shusei Co., Ltd.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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Dispase, a neutral protease isolated from culture filtrates of Bacillus polymyxa, has proven to be a rapid, effective, but gentle agent for separating intact epidermis from the dermis and intact epithelial sheets in culture from the substratum. In both cases
문서
Enzyme Explorer Key Resource: Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
관련 콘텐츠
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
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