SCM141
PLTMax Human Platelet Lysate
PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. 100ml.
동의어(들):
hPL, hPL Human Platelet Lysate Supplement
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모든 사진(5)
About This Item
UNSPSC 코드:
12352207
eCl@ss:
32160801
NACRES:
NA.75
추천 제품
생물학적 소스
human
Quality Level
형태
liquid
제조업체/상표
Chemicon®
기술
cell culture | stem cell: suitable
입력
sample type mesenchymal stem cell(s)
배송 상태
dry ice
일반 설명
PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. Optimal growth of MSCs can be achieved with 5% PLTMax Human Platelet Lysate. PLTMax Human Platelet Lysate has been used to effectively to grow mesenchymal stem cells derived from other species including mouse, rabbit and porcine.
PLTMax Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Heparin (porcine) approved for human intravenous use is used in the preparation of PLTMax Human Platelet Lysate. This product contains human derived materials and must be treated as potentially infectious. Universal precautions for biological samples should be used in handling and disposal.
PLTMax Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Heparin (porcine) approved for human intravenous use is used in the preparation of PLTMax Human Platelet Lysate. This product contains human derived materials and must be treated as potentially infectious. Universal precautions for biological samples should be used in handling and disposal.
애플리케이션
Instructions for Use
• Add Heparin to the media at a final concentration of 2 U/mL. Failure to add heparin may result in coagulation of certain media.
• For optimal growth of human mesenchymal stem cells, add 5% vol/vol PLTMax Human Platelet Lysate to usual cell culture medium such as DMEM and αMEM.
• Plate cells at approximately 1.5x103 cells per cm2.
• Do not allow primary MSC confluence to exceed 80%.
Particulate Formation
• Product has been aseptically filtered with 0.2 µm filters. Particulates will be observed after thawing. Particulate formation is normal will not affect performance even in the presence of large particulates. Filtration is not recommended.
• To reduce particulates from forming, minimize freeze/thaw cycles and extended storage at 4°C. Some particulate formation can be reversed with brief incubation at 37°C, if desired.
• Add Heparin to the media at a final concentration of 2 U/mL. Failure to add heparin may result in coagulation of certain media.
• For optimal growth of human mesenchymal stem cells, add 5% vol/vol PLTMax Human Platelet Lysate to usual cell culture medium such as DMEM and αMEM.
• Plate cells at approximately 1.5x103 cells per cm2.
• Do not allow primary MSC confluence to exceed 80%.
Particulate Formation
• Product has been aseptically filtered with 0.2 µm filters. Particulates will be observed after thawing. Particulate formation is normal will not affect performance even in the presence of large particulates. Filtration is not recommended.
• To reduce particulates from forming, minimize freeze/thaw cycles and extended storage at 4°C. Some particulate formation can be reversed with brief incubation at 37°C, if desired.
PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. 100ml.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
Mesenchymal Stem Cells
품질
PLTMax Human Platelet Lysate is manufactured with the highest quality of raw materials. All human derived materials are obtained from government inspected facilities and are of US origin. Each donor unit was approved for human use and has been tested for infectous diseases including HIV-1, HIV-2, HCV, HBsAg, and RPR for Syphilis.
Each lot is extensively tested for the following parameters:
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Endotoxin USP <8>: <10 EU/mL
Mycoplasma: Not detected
Sterility Testing: Negative for growth
Each lot is extensively tested for the following parameters:
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Endotoxin USP <8>: <10 EU/mL
Mycoplasma: Not detected
Sterility Testing: Negative for growth
저장 및 안정성
Store at -80°C upon receipt. Thaw at room temperature or in a water bath. After thawing, aliquot and store at -20°C or colder. Long term storage at -80°C is recommended. Multiple freeze/thaw cycles are not recommended.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Adrian Gericke et al.
RSC advances, 9(39), 22531-22539 (2019-07-22)
Impaired regeneration of the corneal epithelium, as found in many ocular surface diseases, is a major clinical problem in ophthalmology. We hypothesized that corneal epithelial regeneration can be promoted by the physiological, energy-delivering as well as "morphogenetically active" polymer, inorganic
Mark Li et al.
Stem cells translational medicine, 10(3), 385-397 (2020-10-25)
To optimize the regenerative proficiency of stem cells, a cardiopoietic protein-based cocktail consisting of multiple growth factors has been developed and advanced into clinical trials for treatment of ischemic heart failure. Streamlining the inductors of cardiopoiesis would address the resource
문서
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.
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