추천 제품
제품명
BLaER1 Human B-cell Precursor Leukemia Cell Line, BLaER1 human B-cell precursor leukemia cell line may be transdifferentiated and used to model human monocytes.
생물학적 소스
human
기술
cell culture | mammalian: suitable
배송 상태
ambient
일반 설명
Induction of differentiation is a therapeutic approach for cancer. Treatment of leukemias with differentiation-inducing factors in combination with pro-apoptotic compounds has been shown to be highly effective . Ectopic expression of the transcription factor C/EBPa causes reprogramming of leukemia cells, resulting in expression of a normal cell phenotype.
The BLaER1 cell line is a tamoxifen-inducible derivative of the RCH-ACV B-cell leukemia cell line genetically modified using a retrovirus vector to express C/EBPa fused with the estrogen receptor hormone binding domain (ER) and GFP . A study demonstrated that BLaER1 cells could efficiently convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages . Cells retained their phenotype even when C/EBPa was inactivated, a characteristic of genuine cell reprogramming. C/EBPa induction also impaired cell tumorigenicity after transplantation into immunodeficient mice. BLaER1 is thus a model for highly efficient transdifferentiation of B-cell-derived human cancer cells.
Source:
BLaER1 cells are a single subclone isolated from C/EBPaER-GFP-transfected RCH-ACV acute lymphoblastic leukemia (ALL) cells sorted for GFP expression. The parental cell line was obtained from the bone marrow of a female with chromosome translocation 1;19 and trisomy 8 .
Biosafety Level 2:
BLaER1 cells contain squirrel monkey retrovirus (SMRV) and should be handled under biosafety level 2.
The BLaER1 cell line is a tamoxifen-inducible derivative of the RCH-ACV B-cell leukemia cell line genetically modified using a retrovirus vector to express C/EBPa fused with the estrogen receptor hormone binding domain (ER) and GFP . A study demonstrated that BLaER1 cells could efficiently convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages . Cells retained their phenotype even when C/EBPa was inactivated, a characteristic of genuine cell reprogramming. C/EBPa induction also impaired cell tumorigenicity after transplantation into immunodeficient mice. BLaER1 is thus a model for highly efficient transdifferentiation of B-cell-derived human cancer cells.
Source:
BLaER1 cells are a single subclone isolated from C/EBPaER-GFP-transfected RCH-ACV acute lymphoblastic leukemia (ALL) cells sorted for GFP expression. The parental cell line was obtained from the bone marrow of a female with chromosome translocation 1;19 and trisomy 8 .
Biosafety Level 2:
BLaER1 cells contain squirrel monkey retrovirus (SMRV) and should be handled under biosafety level 2.
세포주 설명
Cancer Cells
애플리케이션
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
품질
- Each vial contains ≥1×10 viable cells.
- Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
- Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
- Cells are negative for mycoplasma contamination.
- Cells tested positive for squirrel monkey retrovirus (SMRV).
저장 및 안정성
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
C/EBPα Induces Highly Efficient Macrophage Transdifferentiation of B Lymphoma and Leukemia Cell Lines and Impairs Their Tumorigenicity.
Francesca Rapino et al.
Cell reports, 19(6), 1281-1281 (2017-05-13)
Grégoire Stik et al.
Nature genetics, 52(7), 655-661 (2020-06-10)
Three-dimensional organization of the genome is important for transcriptional regulation1-7. In mammals, CTCF and the cohesin complex create submegabase structures with elevated internal chromatin contact frequencies, called topologically associating domains (TADs)8-12. Although TADs can contribute to transcriptional regulation, ablation of
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