추천 제품
product name
SF7761 Human DIPG H3.3-K27M Cell Line, SF7761 pediatric diffuse intrinsic pontine glioma (DIPG) cell line harbors the histone H3.3 Lys 27-to-methionine (K27M) mutation and can support research and drug development efforts targeting DIPG.
생물학적 소스
human
Quality Level
기술
cell culture | mammalian: suitable
일반 설명
Diffuse intrinsic pontine gliomas (DIPG) are highly aggressive and difficult to treat tumors arising in the ventral pons of the brain stem. Despite therapeutic advances, DIPG is incurable and most patients, primarily children, die within 2 years of diagnosis. DIPG is one of the leading causes of death in children with brain tumors .
A somatic mutation of histone H3.3 resulting in a lysine 27 to methionine substitution (H3.3K27M) occurs in 60% of DIPG . In H3.3K27M DIPG patient samples, levels of H3K27 dimethylation (H3K27me2) and trimethylation (H3K27me3) are reduced globally. Expression of H3.3K27M was also shown to be associated with increased levels of H3K27 acetylation (H3K27ac) and recruitment of bromodomain proteins at sites of active transcription . These epigenetic changes are thought to be important factors driving DIPG oncogenesis
A somatic mutation of histone H3.3 resulting in a lysine 27 to methionine substitution (H3.3K27M) occurs in 60% of DIPG . In H3.3K27M DIPG patient samples, levels of H3K27 dimethylation (H3K27me2) and trimethylation (H3K27me3) are reduced globally. Expression of H3.3K27M was also shown to be associated with increased levels of H3K27 acetylation (H3K27ac) and recruitment of bromodomain proteins at sites of active transcription . These epigenetic changes are thought to be important factors driving DIPG oncogenesis
SF7761 is a human glioma cell line derived by surgical biopsy from a young female H3.3K27M DIPG patient (5). The tumor cells were immortalized with hTERT (human telomerase ribonucleoprotein reverse transcriptase) using retroviral transduction. SF7761 cells are tumorigenic in athymic rodents with the tumor cells recapitulating the infiltrative growth of human brainstem gliomas (5, 6).
IMPORTANT NOTE: SF7761 are grown as neurospheres in suspension culture. Neurospheres are to be passaged using gentle mechanical trituration when they reach diameters of 200-500 um or through enzymatic dissociation.
IMPORTANT NOTE: SF7761 are grown as neurospheres in suspension culture. Neurospheres are to be passaged using gentle mechanical trituration when they reach diameters of 200-500 um or through enzymatic dissociation.
세포주 설명
Cancer Cells
애플리케이션
Research Category
Cancer
Oncology
Cancer
Oncology
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
품질
• Each vial contains ≥ 200 viable neurospheres.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, C, Herpesvirus type 6, 7, 8 and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, C, Herpesvirus type 6, 7, 8 and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
저장 및 안정성
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Kui-Ming Chan et al.
Genes & development, 27(9), 985-990 (2013-04-23)
Recent studies have identified a Lys 27-to-methionine (K27M) mutation at one allele of H3F3A, one of the two genes encoding histone H3 variant H3.3, in 60% of high-grade pediatric glioma cases. The median survival of this group of patients after
Rintaro Hashizume et al.
Journal of neuro-oncology, 110(3), 305-313 (2012-09-18)
Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain
Peter W Lewis et al.
Science (New York, N.Y.), 340(6134), 857-861 (2013-03-30)
Sequencing of pediatric gliomas has identified missense mutations Lys27Met (K27M) and Gly34Arg/Val (G34R/V) in genes encoding histone H3.3 (H3F3A) and H3.1 (HIST3H1B). We report that human diffuse intrinsic pontine gliomas (DIPGs) containing the K27M mutation display significantly lower overall amounts
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