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Merck
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Key Documents

RADPKMAG-80K

Millipore

MILLIPLEX® Rat Adipokine Panel - Metabolism Assay

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About This Item

UNSPSC 코드:
12161503
eCl@ss:
32161000
NACRES:
NA.84

종 반응성

rat

Quality Level

제조업체/상표

Milliplex®

assay range

accuracy: 94-119%
sensitivity: 0.3-17 pg/mL
(MinDC)

standard curve range: 0.7-3,000 pg/mL
(IL-1β, TNFα)

standard curve range: 12-50,000 pg/mL
(PAI-1)

standard curve range: 15-60,000 pg/mL
(IL-6, Leptin)

standard curve range: 24-100,000 pg/mL
(Insulin)

standard curve range: 9.8-40,000 pg/mL
(MCP-1)

기술

multiplexing: suitable

검출 방법

fluorometric (Luminex xMAP)

배송 상태

wet ice

일반 설명

In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.

MILLIPLEX® Rat Adipokine Magnetic Bead Panel is a seven-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism
The MILLIPLEX® MAP Rat Adipokine Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1 Total, and TNFα.

특이성

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

애플리케이션

  • Analytes: IL-1β, IL-6, Insulin, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
  • Recommended Sample Type: Rat serum or plasma
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
  • Assay Run Time: Overnight (16-18) hours at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine; Obesity; Metabolic Disease

특징 및 장점

Design your multiplex kit by choosing available analytes within this panel.

포장

Everything you need in a single kit.

성분

Rat Adipokine Standard, 1 vial, lyophilized

Rat Adipokine Quality Control 1, 1 vial, lyophilized

Rat Adipokine Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Bead Diluent, 1 vial, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Adipokine Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

저장 및 안정성

Recommended storage for kit components is 2 - 8°C.

기타 정보

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.

법적 정보

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

픽토그램

Skull and crossbonesEnvironment

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


시험 성적서(COA)

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Jaroslava Trnovska et al.
International journal of molecular sciences, 22(19) (2021-10-14)
(1) Background: empagliflozin, sodium-glucose co-transporter 2 (SGLT-2) inhibitor, is an effective antidiabetic agent with strong cardio- and nephroprotective properties. The mechanisms behind its cardio- and nephroprotection are still not fully clarified. (2) Methods: we used male hereditary hypertriglyceridemic (hHTG) rats
Lin Song et al.
Biology of reproduction, 102(3), 705-716 (2019-11-20)
Maternal high-fat (HF) diet negatively affects maternal metabolism and placental function. This study aimed to determine whether gestational exercise prevents the effect of HF diet on placental amino acid transporter expression and nutrient-sensing signaling and the fetal response. Pregnant Sprague-Dawley

관련 콘텐츠

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

Learn the benefits of using biomarker detection multiplex immunoassays for laboratory, companion, and agricultural animal research and explore MILLIPLEX® multiplex assays for veterinary medicine and animal health research.

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