추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
9E10, monoclonal
형태
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
rat (weakly), human, mouse (weakly)
제조업체/상표
Calbiochem®
저장 조건
do not freeze
동형
IgG1
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... MYC(4609)
mouse ... Myc(17869)
rat ... Myc(24577)
일반 설명
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.
Recognizes the ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.
This Anti-c-Myc (Ab-1) Mouse mAb (9E10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Chromatin IP, Paraffin Sections for the detection of c-Myc (Ab-1).
면역원
Epitope: within amino acids 410-419
Human
a synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
애플리케이션
Flow Cytometry (20 µg/ml or use Cat. No. OP10F)
Frozen Sections (2-10 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)
Immunoprecipitation (1 µg/sample)
Chromatin Immunoprecipitation (5 µg/ml, see application references)
Paraffin Sections (not recommended)
Frozen Sections (2-10 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)
Immunoprecipitation (1 µg/sample)
Chromatin Immunoprecipitation (5 µg/ml, see application references)
Paraffin Sections (not recommended)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
분석 메모
Negative Control
HT1080 cells
HT1080 cells
Positive Control
HL-60 cells or lung carcinoma
HL-60 cells or lung carcinoma
기타 정보
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.
The c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a 45 kDa protein, but c-Myc migrates under reducing conditions as a 64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra 34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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