NE1004

Anti-Apolipoprotein E Mouse mAb (E6D7)

liquid, clone E6D7, Calbiochem^®

• 동의어(들): Anti-ApoE
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: E6D7, monoclonal
• 양식: liquid
• 미포함: preservative
• 종 반응성: human
• 반응하면 안 됨: mouse, rabbit
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 동형: IgG1
• 배송 상태: wet ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... APOE(348)

설명

일반 설명

Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.

Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.

면역원

Human

a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE

애플리케이션

ELISA (see comments)
Immunoblotting (1:100-1:1000)
Immunoprecipitation (see comments)
Frozen Sections (1:100-1:10,000)
Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

물리적 형태

Culture supernatant diluted in PBS.

재구성

Following initial thaw, aliquot and freeze (-20°C).

분석 메모

Positive Control
Brain tissue, serum

기타 정보

Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable