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Merck
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주요 문서

MHA000P2N

Millipore

Wallerstein Nutrient Broth

ready-to-use, capacity 2 mL, suitable for microbiology, for bacteria, for molds, for yeasts

동의어(들):

WL Nutrient broth, WLNB, Wallerstein Laboratory Nutrient Broth

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About This Item

UNSPSC 코드:
41106225
eCl@ss:
32160801
NACRES:
NA.74

무균

sterile

Quality Level

양식

liquid

유통기한

12 mo.

특징

ready-to-use

포장

pack of 50 tests

용량

2 mL

filling volume

2 mL

색상

slightly opalescent green-blue

입력

sample type bottled water
sample type beer
sample type sport drink
sample type cider
sample type food(s)
sample type beverage(s)
sample type wine
sample type cosmetics
sample type soft drink
sample type beverage(s)

pH

5.5±0.2 (25.0 °C)

응용 분야

environmental
food and beverages
industrial qc

microbiology

호환성

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

배송 상태

wet ice

저장 온도

2-8°C

적합성

bacteria
molds
yeasts

일반 설명

Wallerstein Nutrient Broth is recommended for the isolation and cultivation of yeasts, molds and bacteria that grow in a low pH environment mainly in the beverage industry. Yeast extract in the medium is a source of trace elements, vitamins and amino acids. Peptone provides nitrogen, amino acids and carbon. Dextrose is a source of carbohydrates. Monopotassium phosphate buffers the media. Potassium chloride, calcium chloride, and ferric chloride are essential ions to help to maintain osmotic balance. Magnesium sulfate and manganese sulfate are sources of divalent cations. Bromocresol green is the pH indicator. The WL Nutrient medium plate incubated under aerobic conditions enables the growth of mainly yeast colonies, acetic acid bacteria, flavobacterium, proteus and thermophilic bacteria and under anaerobic condition enables the growth of lactic acid bacteria and Pediococcus. Bacterial colonies appear as non-pigmented to white, with a smooth or rough texture. Yeast appears as large creamy to white colonies.

애플리케이션

Wallerstein Nutrient Broth is used in the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeast and bacteria. It is used in the beverage industry to detect bacteria, yeast, and mold in wines, beer and other fermentation products.

특징 및 장점

  • Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
  • They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

규격

Incubation Temperature: 35 °C ± 2 for bacteria; 30 °C ± 2 for yeasts and molds
Incubation Time: 48–72 hrs

분석 메모

Bacterial colonies can appear non-pigmented to white, with a smooth or rough texture. Yeast appear as creamy, white larger colonies
QC organisms
L. fermentum (ATCC 9338)

S. cerevisiae (ATCC 9763)
Target Microorganisms: Lactic acid bacteria, yeast and mold

법적 정보

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Media for Isolation-Identification-Cultivation-Maintenance of Medical Bacteria
MacFaddin JF
Williams and Wilkins, 12, 353-353 (1985)
Paper read at American Society of Brewing Chemists Meeting
Green SR and Gray PP
Wallerstein Laboratories Communications, 12, 353-353 (1950)

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