MABS87

Anti-GPR177 Antibody, clone YJ5

clone YJ5, from mouse

• 동의어(들): Protein wntless homolog, Integral membrane protein GPR177, Protein evenness interrupted homolog, Putative NF-kappa-B-activating protein 373, WLS, EVI
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: YJ5, monoclonal
• 종 반응성: human
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001002292
• UniProt 수납 번호: Q5T9L3
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... WLS(79971)

설명

일반 설명

Gpr177 (WLS, EVI), the mouse ortholog of Drosophila WIs, is expressed during mouse embryogenesis. It is essential for the patterning of the anterior-posterior axis during mammalian development and is activated by â-catenin and LEF/TCF-dependent transcription. Upon GPR177 activation, Wnt binds with and modifies the cellular distribution of GPR177, leading to a feedback regulatory mechanism involving Wnt expression and signaling.

면역원

Histidine-tagged recombinant protein corresponding to human GPR177.

애플리케이션

Anti-GPR177 Antibody, clone YJ5 is an antibody against GPR177 for use in WB & IC.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GPR177 in HeLa cells (Prof. D.M. Virshup, Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School.) (Also reference Coombs, G.S., et al. (2010). J Cell Sci. 123(19):3357-3367).

Research Category
Signaling

Research Sub Category
Developmental Signaling

품질

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected GPR177 in 10 µg of HeLa cell lysate.

표적 설명

~62 kDa observed

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
HeLa cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable