540221

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

• 동의어(들): PNP
• UNSPSC 코드: 12352202
• NACRES: NA.42

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 형태: liquid
• 특이 활성도: ≥25 units/mg protein
• 제조업체/상표: Calbiochem^®
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. This reaction is reversible. The human enzyme is a target for therapeutic immune response intervention.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P_i into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

물리적 형태

In 50 mM Tris-HCl, pH 7.6.

분석 메모

Single band by SDS-PAGE

기타 정보

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable