540221

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

• 동의어(들): PNP
• UNSPSC 코드: 12352202
• NACRES: NA.42

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 양식: liquid
• 특이 활성도: ≥25 units/mg protein
• 제조업체/상표: Calbiochem^®
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P_i into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

물리적 형태

In 50 mM Tris-HCl, pH 7.6.

분석 메모

Single band by SDS-PAGE

기타 정보

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable