추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
6G1, monoclonal
종 반응성
rat, mouse
기술
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
mouse ... Nr3C2(110784)
일반 설명
Mineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.
특이성
Epitope is present in all 3 alternative spliced isoforms.
면역원
Conjugated linear peptide corresponding to rat Mineralocorticoid Receptor.
애플리케이션
Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 6G1 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.
Immunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in various rat tissues including kidney, hippocampus, choroid plexus, cerebellum, colon, heart, and coronary artery (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney cells (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected Mineralocorticoid Receptor in rat hippocampal tissue lysate (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney and exogenously expressed human MR in transfected COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney cells (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected Mineralocorticoid Receptor in rat hippocampal tissue lysate (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney and exogenously expressed human MR in transfected COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671).
품질
Evaluated by Western Blotting in M1MR cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of M1MR cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of M1MR cell lysate.
표적 설명
~107 kDa observed
물리적 형태
Format: Purified
기타 정보
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Naoual Dahmana et al.
International journal of pharmaceutics, 604, 120773-120773 (2021-06-07)
Impaired wound healing in patients receiving glucocorticoid therapy is a serious clinical concern: mineralocorticoid receptor (MR) antagonists can counter glucocorticoid-induced off-target activation of MR receptors. The aim of this study was to investigate the cutaneous delivery of the potent MR
Viren H Makhijani et al.
Neuropharmacology, 181, 108337-108337 (2020-10-03)
The mineralocorticoid receptor (MR) is an emerging target in the field of alcohol research. The MR is a steroid receptor in the same family as the glucocorticoid receptor, with which it shares the ligand corticosterone in addition to the MR
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