MABS1982

Anti-TfR1 Antibody, clone 3B82A1

clone 3B82A1, from mouse

• 동의어(들): Transferrin receptor protein 1, TR:TfR1, Trfr, T9, p90, Cd71
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 3B82A1, monoclonal
• 종 반응성: human
• 기술: flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001121620.1
• UniProt 수납 번호: P02786
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TFRC(7037)

설명

일반 설명

Transferrin receptor protein 1 (UniProt: P02786; also known as TR, TfR, TfR1, Trfr, T9, p90, CD71) is encoded by the TFRC gene (Gene ID: 7037) in human. Tfr is a disulfide-bonded homodimeric type II transmembrane glycoprotein that is involved in iron uptake. TfR is more abundantly expressed in rapidly dividing cells. Higher expression levels of TfR are seen in the placental trophoblast and hemoglobin-synthesizing reticulocytes, but expression is lost in mature erythrocytes. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. Both TfR and TfR2 bind diferric transferrin better than apo-transferrin at neutral pH. However, TFR2 displays about 25-fold lower affinity for transferrin compared to TfR. TfR levels are regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3′-UTR. Up-regulated upon mitogenic stimulation. TfR can be N- and O-glycosylated, phosphorylated and palmitoylated, however, the serum form is only glycosylated. TfR can be proteolytically cleaved on Arg100 to produce the soluble serum form (sTfR). Mutations in TFRC gene result in immunodeficiency 46, an autosomal recessive disorder that is characterized by early-onset chronic diarrhea, recurrent infections, hypo- or agammaglobulinemia, intermittent neutropenia, and thrombocytopenia.

특이성

Clone 3B82A1 specifically detects transferrin receptor protein 1. It targets an epitope in the extracellullar domain.

면역원

Epitope: extracellular domain

His-tagged recombinant ectodomain of human Transferrin receptor protein 1.

애플리케이션

Detects Transferrin receptor protein 1 using this mouse monoclonal Anti-TfR1, clone 3B82A1, Cat. No. MABS1982 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected TfR1 in Western Blotting applications (Chloupkova, M., et. al. (2010). Blood Cells Mol Dis. 44(1):28-33; Johnson, M.B., et. al. (2004). Blood. 104(13):4287-93; Chen, J., et. al. (2015). J Biol Chem. 290(12):7841-50; Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14).

Immunocytochemistry Analysis: A representative lot detected TfR1 in Immunocytochemistry applications (Johnson, M.B., et. al. (2007). Mol Cell Biol. 18(3):743-54; Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14).

Immunoprecipitation Analysis: A representative lot detected TfR1 in Immunoprecipitation applications (Johnson, M.B., et. al. (2004). Blood. 104(13):4287-93; Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14).

품질

Evaluated by Flow Cytometry in K562 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected TfR1 in 1X10E6 K562 cells.

표적 설명

84.87 kDa calculated.

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1