추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
BD24, monoclonal
종 반응성
human, pig, avian, bovine
반응하면 안 됨
feline, rat
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... GABRA1(2554)
특이성
Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat
면역원
Epitope: N-terminus
Purified GABA/benzodiazepine receptor from bovine cortex.
애플리케이션
Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB.
Immunohistochemistry: 10-20 μg/mL * See protocol below.
Western blot: 20 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB339
IMMUNOHISTOCHEMISTRY
1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.
2) Sections should be incubated floating in suitable small vials.
3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.
4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).
5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C
6) Wash sections in Buffer A.
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in Buffer A.
9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.
Western blot: 20 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB339
IMMUNOHISTOCHEMISTRY
1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.
2) Sections should be incubated floating in suitable small vials.
3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.
4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).
5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C
6) Wash sections in Buffer A.
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in Buffer A.
9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
물리적 형태
Format: Purified
Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
저장 및 안정성
Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Lisa L Gomez et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(16), 7027-7044 (2002-08-15)
At the postsynaptic membrane of glutamatergic synapses, the cAMP-dependent protein kinase (PKA), protein kinase C (PKC), and calcineurin (CaN) anchoring protein AKAP79/150 is recruited to NMDA and AMPA glutamate receptors by postsynaptic density (PSD)-95 family membrane-associated guanylate kinase (MAGUK) scaffold
Novel ?1 and ?2 GABAA receptor subunit mutations in families with idiopathic generalized epilepsy.
Pamela Lachance-Touchette,Patricia Brown,Caroline Meloche,Peter Kinirons,Line Lapointe et al.
The European Journal of Neuroscience null
Differential localization of GABAA receptor subunits within the substantia nigra of the human brain: an immunohistochemical study.
H J Waldvogel,K Baer,W-P Gai,R T Gilbert,M I Rees,H Mohler,R L M Faull
The Journal of Comparative Neurology null
Ivan Milenkovic et al.
Brain structure & function, 223(3), 1501-1518 (2017-11-24)
The function, regulation and cellular distribution of GABAA receptor subunits have been extensively documented in the adult rodent brain and are linked to numerous neurological disorders. However, there is a surprising lack of knowledge on the cellular (sub-) distribution of
Cell type-specific effect of hypoxia and platelet-derived growth factor-BB on extracellular matrix turnover and its consequences for lung remodeling.
Karakiulakis, G; Papakonstantinou, E; Aletras, AJ; Tamm, M; Roth, M
The Journal of Biological Chemistry null
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