MABN633

Anti-a-Synuclein, clone 10D2 Antibody

clone 10D2, from mouse, purified by affinity chromatography

• 동의어(들): Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 10D2, monoclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_000336
• UniProt 수납 번호: P37840
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SNCA(6622)

설명

일반 설명

α-Synuclein, also known as Non-A beta component of AD amyloid or Non-A4 component of amyloid, or NACP, and encoded by the gene SNCA/NACP/PARK1, is a protein originally identified as non-Abeta component protein (NACP) in the amyloid enriched fraction isolated from Alzheimer’s brain tissue. α-Synuclein is expressed in neural tissue and non-neural tissues too. In neurons, α-Synuclein is localized to the nucleus, presynaptic termini and mitochondria of certain brain regions. α-Synuclein is a molecular chaperone that binds various proteins and compounds including synaptobrevin-2 protein and membrane phospholipids and is involved in vesicle trafficking and membrane transport within neurons. Natural, non-mutated α-Synuclein has autoproteolytic activity and naturally forms stable alpha helical tetramers. However, mutated α-Synuclein can aggregate to form insoluble pathological complexes such as Lewy Bodies inside neurons. Lewy Bodies also contain ubiquitin and it is thought that they are evidence of disrupted proteasome function with regards to α-synuclein but research is still continuing.
This antibody can be used as a sandwich ELISA pair with Cat. No. MABN389, Anti-Aggregated a-Synuclein, clone 5G4.

면역원

Recombinant protein corresponding to human Synuclein.

애플리케이션

Anti-a-Synuclein, clone 10D2 Antibody is an antibody against a-Synuclein for use in western blotting, IHC & ELISA.

Immunohistochemistry Analysis: A representative lot detected aggregated a-Synuclein in human brain tissues.

ELISA Analysis: A representative lot from an independent laboratory detected aggregated a-Synuclein in a sandwich ELISA with Cat. No. MABN389, Anti-Aggregated a-Synuclein, clone 5G4 (Kovacs, G. G., et al. (2012). Acta Neuropathol. 123(1):37-50.).

품질

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.5 µg/mL of this antibody detected a-Synuclein in 10 µg of human brain tissue lysate.

표적 설명

~15 kDa observed

결합

Replaces: 04-1053

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable