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Merck
모든 사진(1)

주요 문서

MABN2292

Sigma-Aldrich

Anti-Neuregulin-1 Antibody, clone 4F10

clone 4F10, from rat

동의어(들):

NRG1, Acetylcholine receptor-inducing activity, ARIA, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, HRG, Neu differentiation factor, Sensory and motor neuron-derived factor

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rat

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

4F10, monoclonal

종 반응성

human

종 반응성(상동성에 의해 예측)

mouse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)

포장

antibody small pack of 25 μg

기술

western blot: suitable

동형

IgG2bκ

NCBI 수납 번호

UniProt 수납 번호

타겟 번역 후 변형

unmodified

유전자 정보

human ... NRG1(3084)

일반 설명

Neuregulin-1 (UniProt: Q02297; also known as Acetylcholine receptor-inducing activity, ARIA, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, HRG, Neu differentiation factor, Sensory and motor neuron-derived factor) is encoded by the NRG1 (also known as GGF, HGL, HRGA, NDF, SMDF) gene (Gene ID: 3084) in human. Neuregulin-1 is a single-pass type 1 membrane protein that acts as a direct ligand for ErbBB3 and ErbB4 tyrosine kinase receptors. It concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Eleven isoforms of Neuregulin-1 are known that are produced by alternative splicing. They have been classified as type I (isoforms with an Ig domain and a glycosylation domain) type II NRGs (isoforms with an Ig domain, but no glycosylation domain). These multiple isoforms perform diverse cellular functions, such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells and inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction. Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. Dual cleavage of Neuregulin-1 by beta-secretase 1 (BACE1) and ADAM17 is shown to liberate EGF-like domain, which stimulates ErbB3 signaling. (Ref.: Fleck, D., et a. (2013). J. Neurosci. 33(18):7856-7869).

특이성

Clone 4F10 is a rat monoclonal antibody that detects human Neuregulin-1. It targets an epitope within 10 amino acids from the N-terminal half.

면역원

A linear peptide corresponding to 10 amino acids from the N-terminal, extracellular domain of human Neuregulin 1, isoform 6.
Epitope: extracellular domain

애플리케이션

Anti-Neuregulin-1 clone 4F10, Cat. No. MABN2292, is a highly specific rat monoclonal antibody that targets Typ III NRG1 beta-1 and has been tested for use in Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: A representative lot detected Neuregulin-1 in Western Blotting applications (Fleck, D., et. al. (2013). J Neurosci. 33(18):7856-69; Cheret, C., et. al. (2013). EMBO J. 32(14):2015-28; Fleck, D., et. al. (2016). J Biol Chem. 291(1):318-33; Ben Halima, S., et. al. (2016). Cell Rep. 14(9):2127-2141).

품질

Evaluated by Western Blotting in HEK293 cells transfected with Type I NRG1 and Type III NRG1 and treated with BACE inhibitor.

Western Blotting Analysis: 1 µg/mL of this antibody detected Neuregulin-1 in HEK293 cells transfected with Type I NRG1 and Type III NRG1 and treated with BACE inhibitor.

표적 설명

~ 71 kDa observed; 71.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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