MABN125

Anti-DRD4 Antibody, clone 2B9

clone 2B9, from mouse

• 동의어(들): D(4) dopamine receptor, D(2C) dopamine receptor, Dopamine D4 receptor
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 2B9, monoclonal
• 종 반응성: human, rat, mouse
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_000788
• UniProt 수납 번호: P21917
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Drd4(13491)

설명

일반 설명

Five subtypes of dopamine receptors exist in two subfamilies: two D1-like (D1 and D5) and three D2-like (D2, D3 and D4). As members of the G protein-coupled receptor (GPCR) family, dopamine receptors initiate their biological function by coupling to GTP regulatory proteins (G proteins). D1-like receptors, DRD1 and DRD5, stimulate adenylyl cyclase whereas D2-like receptors, DRD2, DRD3 and DRD4, are coupled to inhibitory G proteins and their stimulation leads to the inhibition of adenylyl cyclase activity, and subsequent respective cellular signaling. All five subtypes have seven transmembrane domains, an extracellular amino terminus, and an intracellular carboxyl terminus. Allelic variants of the D4 dopamine receptor (DRD4) are known characterized risk factors of ADHD. It is reported that hypoxia enhances DRD4 promoter activity by several fold. It is also thought that dopamine receptors such as DRD4 are involved in neuroexcitation and in a variety of other neurological and psychiatric disorders.

면역원

Linear peptide corresponding to human DRD4.

애플리케이션

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

This Anti-DRD4 Antibody, clone 2B9 is validated for use in WB, IC for the detection of DRD4.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected DRD4 in 10µg of rat brain tissue lysate.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected DRD4 in Ntera-2 cells and in rat brain neurons. (Wolstencroft, E.C., et al. (2007). J Mol Histol. 38(4):333-340.)

품질

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected DRD4 in 10µg of mouse brain tissue lysate.

표적 설명

~ 48 kDa observed.

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Mouse brain tissue lysate.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable