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Merck
모든 사진(3)

주요 문서

MABN1182

Sigma-Aldrich

Anti-Glutamine Synthetase Antibody, clone 11A3.1

clone 11A3.1, from mouse

동의어(들):

Glutamine synthetase, Glutamate-ammonia ligase, Glutamate decarboxylase, GS

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

11A3.1, monoclonal

종 반응성

human, rat

기술

immunohistochemistry: suitable (paraffin)
western blot: suitable

동형

IgG2aκ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

ambient

타겟 번역 후 변형

unmodified

유전자 정보

human ... GLUL (2752)
rat ... Glul(24957)

일반 설명

Glutamine synthetase (EC 4.1.1.15, EC 6.3.1.2; UniProt P09606; also known as Glutamate-ammonia ligase, Glutamate decarboxylase, GS) is encoded by the Glul (also known as Glns) gene (Gene ID 24957) in rat species. Glutamine synthetase (GS) plays an essential role in nitrogen metabolism by catalyzing ATP-dependent condensation of glutamate and ammonia to form glutamine. Three classes of GS exist, class I enzymes (GSI) are specific to prokaryotes and exist as oligomers of 12 identical subunits, class II enzymes (GSII) exist as decamer of identical subunits in eukaryotes and in some bacteria, while class III enzymes (GSIII) function as a double-ringed dodecamer of identical chains found in bacteroides fragilis and in butyrivibrio fibrisolvens. GS is present predominantly in the brain, kidneys, and liver in mammals. In the brain, GS is found primarily in astrocytes, where it participates in the the detoxification of ammonia via assimilation and termination of neurotransmitter signals via recyclization. c-Myc promotes glutamine usage by upregulating glutaminase (GLS), which converts glutamine to glutamate in the TCA cycle, while in some cancer cells Myc-mediated thymine DNA glycosylase (TDG) transcription leads to GS upregulation due to TDG-dependent demethylation of the GS promoter. GS upregulation promotes cancer cell survival under glutamine limitation, while GS silencing decreases cancer cell proliferation and xenograft tumor growth.

특이성

Clone 11A3.1 targets an internal epitope within the C-terminal half of human and rat glutamine synthetase.

면역원

GST-tagged recombinant rat glutamine synthetase internal fragment.

애플리케이션

Anti-Glutamine Synthetase, clone 11A3.1, Cat. No. MABN1182, is a highly specific mouse monoclonal antibody that targets Glutamine Synthetase, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: A 1:20,000 dilution of from a representative lot detected Glutamine synthetase in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Glutamine synthetase in human cerebellum, cerebral cortex, and tonsil tissue sections.

품질

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Glutamine synthetase in 10 µg of rat brain tissue lysate.

표적 설명

~42 kDa observed. 42.06 kDa (human) and 42.27 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified
Protein G purified.
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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