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Merck
모든 사진(1)

주요 문서

MABF33

Sigma-Aldrich

Anti-MHC class II (I-A/I-E) Antibody, clone M5/114

clone M5/114, from rat

동의어(들):

CD74 molecule, major histocompatibility complex, class II invariant chain, Ia-associated invariant chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), gamma chain of class II antigens, Ia antigen-

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rat

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

M5/114, monoclonal

종 반응성

mouse

기술

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG2bκ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

mouse ... Cd74(16149)

일반 설명

MHC class II-associated invariant chain (I-A/I-E), also known as Ia antigen-associated invariant chain, are single-pass type II membrane glycoproteins containing a single thyroglobulin type-I domain. They are critical components in the processing of MHC class II antigen through forming a stabilizing complex following the alpha/beta heterodimer synthesis, and orchestrating complex trafficking away from the ER compartments in which class II peptide loading occurs.

애플리케이션

Detect MHC class II (I-A/I-E) using this Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 validated for use in WB, FC, IH.
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC on spleen cells. (Bhattacharya, A., et al. (1981). The Journal of immunology. 127(6):2488-2496.)

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH on thymus tissue. (de Bont, E., et al. (1999). Int. Immunol. 11(8):1295-1306.)

품질

Evaluated by Western Blot in mouse spleen tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MHC class II (I-A/I-E) in 10 µg of mouse spleen tissue lysate.

표적 설명

~32 kDa observed.
There are 2 isoforms produced by alternative splicing: 32 kDa Isoform Long and 24 kDa Isoform Short.

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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