추천 제품
생물학적 소스
rat
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
9A10D2, monoclonal
종 반응성
human, mouse
기술
immunohistochemistry: suitable
western blot: suitable
동형
IgG2aκ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... COCH(1690)
일반 설명
The protein Cochlin/COCH is important in the development of the cochlea and vestibule in mammals as well as other functions. Cochlin/COCH is an extracellular matrix protein that plays a role in cell shape, positioning and motility. Recent research shows that it is also important in our innate immune response to bacteria and pathogens. Upon bacterial infection, a fragment of Cochlin/COCH is released from the conduits of B cell follicles in the spleen and this triggers protective cytokines in the periphery. Cochlin/COCH is expressed in epithelial tissues in various tissues, particularly in follicular cells and network of conduits in the spleen and lymph nodes. Cochlin/COCH also is a target for BMP4 mediation in embryonic stem cells (ESCs) where it facilitates self-renewal. The protein also appears to suppress neural differentiation of ESCs and suggests that Cochlin/COCH protein is important in ES cell fate determination. Mutations in the Cochlin/COCH gene are responsible for human DFNA9 syndrome, a disorder characterized by neurodegeneration of the inner ear that leads to hearing loss and vestibular impairments.
면역원
Recombinant protein corresponding to human Cochlin/COCH.
애플리케이션
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Anti-Cochlin Antibody/COCH, clone 9A10D2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Cochlin/COCH.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Cochlin/COCH in 10 µg of human lymph node tissue lysate.
Western Blotting Analysis: A representative lot detected Cochlin/COCH in spleen and lymph node from WT and COCH knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Western Blotting Analysis: A representative lot detected Cochlin/COCH in protein cleaved into p18 and p8 products. (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cochlin/COCH in mouse spleen tissue.
Immunohistochemistry Analysis: A representative lot detected Cochlin/COCH in spleen and lymphnode from WT and Coch knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Western Blotting Analysis: A representative lot detected Cochlin/COCH in spleen and lymph node from WT and COCH knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Western Blotting Analysis: A representative lot detected Cochlin/COCH in protein cleaved into p18 and p8 products. (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cochlin/COCH in mouse spleen tissue.
Immunohistochemistry Analysis: A representative lot detected Cochlin/COCH in spleen and lymphnode from WT and Coch knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
품질
Evaluated by Western Blotting in mouse spleen tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cochlin/COCH in 10 µg of mouse spleen tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cochlin/COCH in 10 µg of mouse spleen tissue lysate.
표적 설명
~59 kDa observed. This protein is cleaved into p18 and p8 products. Uncharacterized bands may be observed in some lysates.
물리적 형태
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Dorien Verdoodt et al.
International journal of molecular sciences, 22(21) (2021-11-14)
Several studies have shown that type IV fibrocytes, located in the spiral ligament, degenerate first after noise exposure. Interestingly, this is the region where Coch expression is most abundant. As it is suggested that cochlin plays a role in our
Cochlin produced by follicular dendritic cells promotes antibacterial innate immunity.
Py, BF; Gonzalez, SF; Long, K; Kim, MS; Kim, YA; Zhu, H; Yao, J; Degauque, N; Villet et al.
Immunity null
Qi Wang et al.
PloS one, 12(1), e0170011-e0170011 (2017-01-19)
By analyzing the different phenotypes of two Chinese DFNA9 families with the same mutation located in the intervening region between the LCCL and vWFA domains of cochlin and testing the functional changes in the mutant cochlin, we investigated the different
Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation.
Vogel, KU; Edelmann, SL; Jeltsch, KM; Bertossi, A; Heger, K; Heinz, GA; Zoller, J; Warth et al.
Immunity null
Daniel T Montoro et al.
Nature, 560(7718), 319-324 (2018-08-03)
The airways of the lung are the primary sites of disease in asthma and cystic fibrosis. Here we study the cellular composition and hierarchy of the mouse tracheal epithelium by single-cell RNA-sequencing (scRNA-seq) and in vivo lineage tracing. We identify
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