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Merck
모든 사진(1)

주요 문서

MABF2302

Sigma-Aldrich

Anti-HLA-A02:01 EBV-LMP2A Antibody, clone MAB243

동의어(들):

Latent membrane protein 2

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.43

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

MAB243 (also referenced as L2), monoclonal

분자량

calculated mol wt 53.01 kDa

정제법

using protein G

종 반응성

human

포장

antibody small pack of 100 μg

기술

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

동형

IgG1κ

에피토프 서열

C-terminal

단백질 ID 수납 번호

UniProt 수납 번호

배송 상태

ambient

타겟 번역 후 변형

unmodified

유전자 정보

human ... LMP2(3783751)

일반 설명

Latent membrane protein 2 (UniProt: P13285; also known as Terminal protein) is encoded by the LMP2 gene (Gene ID: 3783751) in Epstein-Barr virus. EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. In healthy individuals, EBV typically establishes a persistent latent infection in which the virus can be detected in resting, nonproliferating peripheral B-lymphocytes. These latently infected cells express only 2 virally encoded genes, LMP2A and EBNA1. Although the majority of virus infected individuals remain healthy, EBV has been implicated in the pathogenesis of African Burkitt s lymphoma and nasopharyngeal carcinoma. LMP2A, a multi-pass membrane protein, localizes in plasma membrane lipid rafts. It maintains EBV latent infection of B cells by preventing lytic reactivation of the virus in response to surface immunoglobulin (sIg) cross-linking. It acts like a dominant negative inhibitor of the sIg-associated protein tyrosine kinases, LYN and SYK. It is also reported to block the translocation of the B-cell antigen receptor (BCR) into lipid rafts, which prevents the subsequent signaling and accelerated internalization of the BCR upon BCR cross-linking. It also serves as a molecular scaffold to recruit SYK, LYN and E3 protein-ubiquitin ligases leading to ubiquitination and degradation of these tyrosine kinases. LMP2A also exhibits a constitutive signaling activity in non-transformed cells, inducing bypass of normal B lymphocyte developmental checkpoints, which allows immunoglobulin-negative cells to colonize peripheral lymphoid organs. (Ref.: Lai, J., et al. (2017). Sci. Rep. 7; 9923; Rancan, C., et al. (2015). PLoS Pathol. 11(6); e1004906).

특이성

Clone MAB423 (L2) is a mouse monoclonal antibody that detects EBV-LMP2A. It targets an epitope within 9 amino acids from the C-terminal region.

면역원

HLA-A201 with EBV (strain B95-8) LMP2A (CLGGLLTMV) 426-434 recombinant protein.

애플리케이션

Quality Control Testing

Evaluated by Flow Cytometry in T2 cells pulsed with LMP2 peptide (426-434).

Flow Cytometry Analysis (FC): 1 μg of this antibody detected HLA-A2-EBV LMP2A in one million T2 cells pulsed with LMP2 peptide (426-434).

Tested Applications

ELISA Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in ELISA applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Lai, J., et al. (2017). Sci Rep. 7(1):9923).

Immunohistochemistry Applications: A representative lot detected HLA-A02:01 EBV-LMP2A in Immunohistochemistry applications (Lai, J., et al. (2016). Blood. 128(10):1396-407).

Western Blotting Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in Western Blotting applications (Lai, J., et al. (2016). Blood. 128(10):1396-407).

Flow Cytometry Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in Flow Cytometry applications (Lai, J., et al. (2016). Blood.;128(10):1396-407; Lai, J., et al. (2017). Sci Rep.;7(1):9923).

Immunofluorescence Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in Immunofluorescence applications (Lai, J., et al. (2016). Blood. 128(10):1396-407).

Immunocytochemistry Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in Immunocytochemistry applications (Lai, J., et al. (2016). Blood. 128(10):1396-407).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-HLA-A02:01 EBV-LMP2A, clone MAB243, Cat. No. MABF2302, is a mouse monoclonal antibody that detects EBV-LMP2A and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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