MABF2163

Anti-CD33 Antibody, clone P67.6

clone P67.6, from mouse

• 동의어(들): Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: P67.6, monoclonal
• 종 반응성: human
• 포장: antibody small pack of 25 μg
• 기술: flow cytometry: suitable; immunocytochemistry: suitable; inhibition assay: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001763.3
• UniProt 수납 번호: P20138
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CD33(945)

설명

일반 설명

Myeloid cell surface antigen CD33 (UniProt: P20138; also known as Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33) is encoded by the CD33 (also known as SIGLEC3) gene (Gene ID: 945) in human. CD33 is a single-pass type I membrane sialoadhesion protein that is expressed by myeloid stem cells, myeloblasts, monoblasts, monocytes, macrophages, and granulocyte precursors and preferentially binds to alpha-2,6-linked sialic acid. CD33 is not expressed in erythrocytes, platelets, B-cells, T-cells, and NK cells. Hence, it can serve as a marker for myeloid cells. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 18-259), a transmembrane domain (aa 260-282), and a cytoplasmic domain (aa 283-364). CD33 contains two copies of a cytoplasmic motif (aa 338-343 and 356-361), which is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. In the immune response, CD33 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Human CD33 is reported to contain two tyrosine residues in its cytoplasmic domain (tyrosine 340 and 358) that when phosphorylated could function as docking sites for the phosphatases, SHP-1 and/or SHP-2, and enable CD33 to function as an inhibitory receptor.

특이성

Clone P67.6 specifically detects human CD 33.

면역원

FMY9S5 cells expressing CD33.

애플리케이션

Anti-CD33, clone P67.6, Cat. No. MABF2163, is a mouse monoclonal antibody that detects CD33 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition assays.

Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58).

Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).

Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).

Research Category
Inflammation & Immunology

품질

Evaluated by Flow Cytometry in THP-1 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD33 in one milliion THP-1 cells.

표적 설명

39.82 kDa calculated.

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.