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Merck
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Key Documents

MABE343-AF647

Sigma-Aldrich

Anti-Puromycin, clone 12D10, Alexa Fluor 647 Conjugate Antibody

clone 12D10, 0.5 mg/mL, from mouse

동의어(들):

Alexa Fluor 647 Anti-Puromycin, Clone 12D10 Anti-Puromycin, Puromycin Detection Antibody

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

ALEXA FLUOR 647

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

12D10, monoclonal

종 반응성

human

농도

0.5 mg/mL

기술

immunocytochemistry: suitable

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

특이성

Predicted to react with cells from all species when pre-incubated with puromycin.

품질

Evaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

Alexa Fluor is a registered trademark of Life Technologies.

표적 설명

Refer to Cat. No. MABE343 for observed molecular weight information.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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