MABE330

Anti-c-ErbB2/c-Neu Antibody, clone 3B5

clone 3B5, from mouse

• 동의어(들): Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 3B5, monoclonal
• 종 반응성: human
• 기술: immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001005862
• UniProt 수납 번호: P04626
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ERBB2(2064)

설명

일반 설명

The ErbB2 (HER2, c-Neu, ErbB2, c-erbB2, or HER2/neu) protein is member of the human epidermal growth factor receptor (HER) family. It is a receptor tyrosine kinase containing an intracellular kinase domain and an extracellular ligand domain, although no endogenous ligand has yet been found. ErbB2 dimerizes with ErbB1, 3, and 4, and can activate different pathways including the PI3K, PLCγ, and MAPK pathways, depending on the ErbB receptor involved. Coupling with ErbB3 activates the PI3K pathway, whereas coupling with any of these three receptors can induce the MAPK pathway. ErbB2 signaling promotes cell proliferation and inhibits apoptosis. ErbB2 is overexpressed in various cancers and may play a role in the development and metastasis of gliomas, and ovarian, breast, lung, and gastric cancer.

면역원

Linear peptide corresponding human c-ErbB2/c-Neu.

애플리케이션

Anti-c-ErbB2/c-Neu Antibody, clone 3B5 is a Mouse Monoclonal Antibody for detection of c-ErbB2/c-Neu also known as MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2 or CD340, has been validated in WB & IHC.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-ErbB2/c-Neu in human breast cancer tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

품질

Evaluated by Western Blot in SK-BR-3 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-ErbB2/c-Neu in 10 µg of SK-BR-3 cell lysate.

표적 설명

~185 kDa observed. Uniprot describes four isoforms at ~138 kDa (isoform 1), ~63 kDa (isoform 2), ~71 kDa (isoform 3), and ~137 kDa (isoform 4), which can be highly glycosylated and phosphorylated. This antibody may detect one or more isoforms. Uncharacterized bands may be observed at ~27 kDa and ~38 kDa in some cell lysates.

결합

Replaces: 04-1127

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
SK-BR-3 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable